Sudarshan Narasimha Murthy
Oulu
Summary
Accomplished Postdoctoral Researcher with a robust background in biochemistry and molecular medicine, notably at the University of Oulu and Leids Universitair Medisch Centrum. Excelled in developing small-molecule inhibitors and solving protein-ligand structures using Cryo-EM and X-ray crystallography. Skilled in scientific writing and project management, with a proven track record in enhancing research design and classroom instruction.
Overview
11
11
years of professional experience
Work History
Postdoctoral Researcher
University Of Oulu
03.2023 - Current
- Protein Expression, purification, and characterization
- Develop Biochemical Assay, Develop direct MS-based assay
- Solving Protein-ligand Structure Using Cryo-EM
- Drafted manuscripts and presented findings at major conferences (Ex- GRC)
- Collaborated with multidisciplinary team members to accomplish research goals.
- Authored professional scientific papers for publishing in peer-reviewed journals.
- Mentored junior researchers, enhancing their skills and supporting their career development.
- Developed new protocols and improved existing laboratory processes.
Postdoctoral Researcher
Leids Universitair Medisch Centrum (LUMC)
03.2021 - 02.2023
- Develop Small-molecule inhibitors against DnaE1
- Protein Expression, purification and characterization
- Biochemical Assay, High Throughput Screening, Validation
- Solving Protein-ligand Structure Using Cryo-EM
- Cell and Chemical Biology Department
- Authored professional scientific papers for publishing in peer-reviewed journals.
- Contributed to grant applications by conducting preliminary studies that supported project feasibility and relevance.
Postdoctoral Researcher
University Of Oulu
10.2020 - 02.2021
- Develop Small Molecule Inhibitors for Specific mono-PARPs
- Protein Expression, purification and characterization
- Structure solution using X-ray crystallography and SAXS
- Involved in teaching lab courses for bachelor degree program which is provided by University of Oulu
- Co-supervised Master’s thesis and regularly taught Master orientation students
- Drafted manuscripts and presented findings at major conferences.
- Collaborated with multidisciplinary team members to accomplish research goals.
Project Assistant
Indian Institute Of Science
07.2014 - 05.2015
- Company Overview: Centre for Ecological Science
- Phylogeographic studies of Nyctibatrachus, an endemic frog genus from Western Ghats
- Molecular work involving PCR, Sequencing
- Bioinformatic analysis
- Centre for Ecological Science
- Researched and analyzed data to support project-related decisions.
Technical Trainee
Aurigene Discovery Technologies Ltd
10.2013 - 05.2014
- Involved in conducting crystallization experiments- data collection, processing and solving ligand-bound complex protein structures
- Training undergraduate students in their dissertation project which involved setting up protein crystallization, data collection, analysis and structure solution.
- Strengthened communication skills by presenting project updates to management teams during regular progress meetings.
- Collaborated with cross-functional teams on various projects, enhancing teamwork skills and adapting to different work styles.
- Helped streamline repair processes and update procedures for support action consistency.
- Researched and identified solutions to technical problems.
Education
Ph.D. - Human Mono-ADP-Ribosyltransferases - Inhibitor Development For New Potential Drug Targets
University Of Oulu
Oulu, Finland09.2020
Master of Science - Structural studies on Adenylosuccinate Lyase from Salmonella typhimurium
Bangalore University
Bengaluru, India06.2013
Skills
- Scientific Writing
- Protein Chemistry ( Cloning, Expression, Purification, Biochemical and Biophysical Analysis)
- Structural Biology ( X- ray Crystallography and Cryo-EM)
- Drug Discovery ( Assay development, Screening and Development of SAR)
- Computational skills
- Project Management
- Classroom Instruction
- Research design
- Equipment Maintenance
- Teaching experience
- Literature review
Presentations
- Collagen 2023 - Gordon Conference, New London, New Hampshire, USA, Poster Presentation, Structural Studies of the Human Collagen Prolyl 4-hydroxylases
- FORMULATRIX Web series, Crystallographic binding studies of ADP-ribosyl-transferase inhibitors using the Instruct-FI/BCO PX-core facility in Oulu, Finland
- The PARP Family & ADP-Ribosylation 2018, CSHL, New York, USA, Poster presentation, Structure-activity relationship of 4-(phenoxy) and 4-(benzyloxy)-benzamides as inhibitors of mono-ADP-ribosyltransferase PARP10/ARTD10
- PARP2019, Budapest, Hungary, Poster presentation, Development of small-molecule inhibitors against potential cancer drug targets - Human Mono-ADP- ribosyltransferases
- Open-SESAME and Instruct-ERIC workshop, Weizmann Institute of Science, Rehovot, Israel, Poster presentation, Development of small-molecule inhibitors against mARTD members
- FEBS Practical Course: Biomolecules in Action II, Hamburg, Germany, Poster presentation, Development of small-molecule inhibitors against mARTD members
- 2nd Nordic PhD Summit: Think Open, Helsinki, Finland, Poster presentation, Development of small-molecule inhibitors against mARTD members
Fundingreceived
- Personal Grant, Magnus Ehrnroothin Foundation, 11,500 €, 2018
- Personal Grant, Magnus Ehrnroothin Foundation, 11,500 €, 2019
- Personal Grant, University of Oulu, 5,500 €, 2020
- Travel Grant, Weizmann Institute of Science, 2018
- Travel grant, FEBS, 2019
Supervisingandmentoring
- Mentor, Claudia Trujillo Olvera, Career mentoring program
- Co-supervisor, DNA damage sensitization of breast cancer cells with PARP10 inhibitor, Mikko Hukkanen, Actively looking for PhD position
- Trainer, Maria Giulia Nizi, Erasmus exchange student, PhD student at the University of Perugia, Italy
- Trainer, Bachelors practical course: Biochemical Methodologies
- Trainer, Elena Rolina - Research technician, Sarah Wazir - PhD student at the University of Oulu, Bao Nguyen - PhD student at the University of Oulu
Publicoutreach
NSMB - Natural Science Meets Business, 2017, Oulu, Finland, Poster Presentation, Development of small molecule inhibitors against mARTDs –PARP10
Reviewer
- Instruct, Scientific Reviewer
- Frontiers in Molecular Biosciences, Reviewer
Orcidid
https://orcid.org/0000-0001-5602-4202
Awards
Best poster presentation, Open-SESAME and Instruct-ERIC workshop, Weizmann Institute of Science, 2018
References
- Prof. Lari Lehtiö, Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine, University of Oulu, Lari.lehtio@oulu.fi
- Prof. Emeritus Rikkert Wierenga, Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine, University of Oulu, Rik.Wierenga@oulu.fi
Patent
OU21018 / PARP inhibitor scaffold OUL40
Timeline
Postdoctoral Researcher
University Of Oulu
03.2023 - Current
Postdoctoral Researcher
Leids Universitair Medisch Centrum (LUMC)
03.2021 - 02.2023
Postdoctoral Researcher
University Of Oulu
10.2020 - 02.2021
Project Assistant
Indian Institute Of Science
07.2014 - 05.2015
Technical Trainee
Aurigene Discovery Technologies Ltd
10.2013 - 05.2014
Ph.D. - Human Mono-ADP-Ribosyltransferases - Inhibitor Development For New Potential Drug Targets
University Of Oulu
Master of Science - Structural studies on Adenylosuccinate Lyase from Salmonella typhimurium
Bangalore University
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